期刊
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
卷 101, 期 6, 页码 2284-2293出版社
WILEY
DOI: 10.1002/jsfa.10849
关键词
Pleurotus pulmonarius; supercritical CO2(SC-CO2) extraction; ultrasound-assisted extraction with water as solvent (UAEW); chemical analysis; antioxidant; enzyme inhibition activity
资金
- Ministry of Education, Science and Technological Development of the Republic of Serbia [45017, 45001]
The study explored the impact of different extraction methods on the chemical profile and bioactivity of Pleurotus pulmonarius. The results showed that supercritical CO2 extraction with co-solvent was the most suitable method for obtaining extracts rich in ergosterol content. Additionally, all types of extracts exhibited significant tyrosinase and amylase inhibition.
Background This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO2) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom,Pleurotus pulmonarius. Results Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO(2)extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g(-1). The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level. Conclusion Based on our results, the extraction methods significantly affected the chemical profile and bioactivity ofP. pulmonarius. (c) 2020 Society of Chemical Industry
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