Efficient removal of ginkgotoxin fromGinkgo bilobaseed powder by combining endogenous enzymatic hydrolysis with resin adsorption

BACKGROUND Ginkgotoxin including 4 '-O-methylpyridoxine (MPN) and MPN-5 '-glucoside (MPNG) is responsible forGinkgoseed food poisoning. The purpose of the work reported was to prepare detoxifiedGinkgoseed powder and at the same time to retain the nutritional and functional components ofGinkgoseed powder to the maximum extent. RESULTS Resin adsorption technology was firstly employed to remove ginkgotoxin from water extract ofGinkgoseed powder. Under optimal conditions, the adsorption efficiency of the optimal resin for MPN could reach 100%, and that for MPNG could only reach 85.4 +/- 0.93%. Resin adsorption alone could not effectively remove MPN and MPNG simultaneously. Endogenous enzymatic hydrolysis was next attempted to transform MPNG to MPN. MPNG could be completely hydrolyzed to MPN by endogenous enzyme(s) at 40 degrees C and pH 5.0 in 180 min. Ginkgotoxin only in the form of MPN in the enzymatic hydrolysate was then adsorbed with resin and the conditions were statistically optimized. The adsorption efficiency of MPN reached 98.89 +/- 0.99% under the optimized conditions. CONCLUSIONS Removal of ginkgotoxin by combining endogenous enzymatic hydrolysis with resin adsorption could preserve the main nutritional and functional components ofGinkgoseed powder to the most extent, and did not change its main characteristics. The ginkgotoxin removal method developed in this work is a relatively simple and efficient approach. (c) 2020 Society of Chemical Industry

Automated summary

The study developed a method combining resin adsorption and endogenous enzymatic hydrolysis to remove ginkgotoxin from Ginkgo seed powder, preserving its main nutritional and functional components to the maximum extent without altering its main characteristics. This approach proved to be relatively simple and efficient.