期刊
JOURNAL OF RAMAN SPECTROSCOPY
卷 52, 期 2, 页码 506-515出版社
WILEY
DOI: 10.1002/jrs.6002
关键词
iron-deficiency anemia detection; SERS-active MOF-AuNP complexes; surface-enhanced Raman scattering; total iron-binding capacity
类别
资金
- National Research Foundation of Korea [2019R1A2C3004375, 2020R1A5A1018052]
- National Research Foundation of Korea [2019R1A2C3004375] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study presents a rapid and sensitive method for determining the total iron-binding capacity of transferrin in human serum using surface-enhanced Raman scattering (SERS) spectroscopy. Metal-organic framework (MOF)-gold nanoparticle (AuNP) complexes were utilized as the SERS substrate to enhance the Raman signals of reporter molecules, allowing for quantitative estimation of Tf concentration. The SERS-based analysis using MOF-AuNPs shows potential for diagnosing iron deficiency in human serum with a detection limit of 0.51 mu M.
We report the rapid and sensitive determination of the total iron-binding capacity of transferrin (Tf) in human serum using surface-enhanced Raman scattering (SERS) spectroscopy. Herein, metal organic framework (MOF)-gold nanoparticle (AuNP) complexes were used as the SERS substrate. The highly porous MOF preconcentrates the reporter molecules and accelerates the approach of the reporter molecules to the vicinity of the AuNPs on the surface of the MOF. As a result, a significant number of reporter molecules are trapped close to SERS-active hot spots, and their Raman signals are greatly enhanced. Additionally, Tf acts as an anchor to form a three-dimensional assembly of MOF-AuNP units via specific binding to the Fe(III) ions of adjacent MOF units when MOF-AuNP complexes are added to human serum. This leads to further enhancement of the Raman signals of Raman reporter molecules with increasing Tf concentration. Quantitative estimation of Tf is possible by monitoring the variations in the intensity of the Raman signal of Raman reporter molecules adsorbed on MOF-AuNP complexes as a function of the Tf concentration. In this work, SERS-based analysis using the MOF-AuNP complexes was performed to determine the concentration of Tf in the human serum for diagnosing iron deficiency. The limit of detection of Tf was determined to be 0.51 mu M in spiked human serum. This SERS-based analysis of Tf using MOF-AuNPs provides new insight for the rapid and sensitive diagnosis of iron deficiency in human serum.
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