期刊
JOURNAL OF PHARMACEUTICAL SCIENCES
卷 110, 期 1, 页码 87-96出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.xphs.2020.10.037
关键词
Quantitative targeted proteomics; Membrane fractionation; Differential detergent fractionation; Drug metabolizing enzymes; Drug transporters; Hepatocytes; Liver
资金
- Innovation in Regulatory Science Award from the Burroughs Wellcome Fund [S10 RR024595]
This study evaluated the efficiency of sequential digitonin and Triton-X-100 fractionation of membrane protein from limited samples for QTAP of DMEs and transporters. Results showed that DDF produced linear membrane protein yields with better permeabilization compared to other methods, making it an efficient and cost-effective approach.
The fractionation of enough membrane protein from limited samples is challenging for MS-based quantitative targeted absolute proteomics (QTAP) of drug metabolizing enzymes (DMEs) and transporters. This study evaluated differential detergent fractionation (DDF) of membrane protein from progressively smaller numbers of primary mouse hepatocytes (5 million down to 50,000 cells) and limited liver tissue (25-50 mg) in quantifying select DMEs and transporters by QTAP. Two non-ionic detergents, digitonin and Triton-X-100, were applied in sequence to permeabilize cells and extract membrane proteins. Comparison was made with a membrane protein extraction kit and with homogenization in hypotonic buffer and subsequent differential centrifugation (DC). DDF produced linear membrane protein yields with increasing hepatocyte numbers and better permeabilization evidenced by the higher ratio of cytosolic to membrane protein yields. DDF produced 5-times more membrane protein from liver tissue than DC. The concentration of DMEs and transporters remained consistent in the fractions prepared by DDF from progressively smaller numbers of hepatocytes, but declined in kit fractions. In liver tissue, the concentrations were comparatively higher in DDF versus kit and DC. In conclusion, sequential digitonin and Triton-X-100 fractionation of membrane protein from limited samples is efficient, reproducible and cost-effective for QTAP of DMEs and transporters. (C) 2020 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved.
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