期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 189, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2020.113471
关键词
Rubia species; Quinones; Analysis; Multiple reaction monitor; Chemometrics
资金
- National Natural Science Foundation of China [21702231, 31900269]
- Natural Science Foundation of Jiangsu Province [BK20170745]
- National New Drug Innovation Major Project of Ministry of Science and Technology of China [2017ZX09309027]
- Program of Innovative Research Team of Jiangsu Province
- Double-First-Class University Project of China Pharmaceutical University [CPU2018GF05]
Rubia species are one of the important multi-origin phytomedicines having both economical and medicinal values in many countries. Quinones are the predominant bioactive constituents of these species. Therefore, accurate analysis of these quinones is critical to quality control, clinical, and commercial applications of Rubia species. In this study, a sensitive and efficient ultra-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-QqQ-MSIMS) method in positive and negative multiple reaction monitor (+/-MRM) modes was firstly developed for the characterization of 45 quinones and the quantification of 24 quinones from Rubia plants. The chromatographic separation was achieved on a Waters ACQUITY UPLC (R) BEH C-18 column by using a gradient elution with a mobile phase consisting of 0.1 % formic acid in water and acetonitrile. The results indicated that quinones in multi-origin Rubia samples were different, but 10 quinones existed in all samples. R. cordifolia, which is a unique official medicinal material in Chinese Pharmacopeia, contained the most number of quinones among all the Rubia species. R. podantha and R. sylvatica had the next highest number of quinones, implying that these species could be used as alternatives for R. cordifolia. Chemometric approaches were applied to evaluate the chemical relationship between the Rubia samples based on the constituent quinones. In this study, a UPLC-QqQ-MSIMS method in the MRM mode has been developed for the analysis of Rubia species-derived quinones, which not only contributes to quality control and discrimination of Rubia species, but also suggests the potential of these species in clinical and commercial applications. (C) 2020 Elsevier B.V. All rights reserved.
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