期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 190, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2020.113511
关键词
Magnetic molecularly imprinted polymers; Human serum albumin; Biomimetic immunoassay; Surfactant-mediated sol-gel method
资金
- National Natural Science Foundation of China [81573391, 81773689, 81603073]
- Shaanxi Province Natural Science Foundation [2017JQ8026]
- China Postdoctoral Science Foundation [2016M602836]
- Postdoctoral Science Foundation of Shaanxi Province [2017BSHYDZZ40]
Molecularly imprinted polymers have demonstrated tremendous potential in the immunoassay as alternatives to biological antibodies. However, the production of molecularly imprinted polymers for protein remains great challenges because of structural complexity and organic solvent instability. In addition, non-specific binding sites in the molecularly imprinted polymers debase the feasibility of it as alternative to antibodies for immunoassay. Here, a surfactant-mediated sol-gel system in an aqueous environment was designed to produce the molecularly imprinted polymers for protein. A blocked strategy was introduced to decrease non-specific cross-reactivity and to improve the selectivity. The developed products were characterized by infrared spectroscopy, scanning electron microscope, transmission electron microscope, X-ray photoelectron spectroscopy, and vibrating sample magnetometer, respectively. The obtained molecularly imprinted polymers exhibited desirable specific recognition towards the target and a biomimetic immunoassay method was developed. The method exhibited a good linear response to human serum albumin in a concentration range of 1-100 mu g mL(-1). The limit of detection of this method was 0.3 mu g mL(-1) (3s/K), and good recoveries ranging from 85.4-104.5% were achieved. This study demonstrated that the molecularly imprinted polymers prepared by the surfactant-mediated sol-gel method can produce high selectivity materials, which had great potential to replace antibodies in a biomimetic immunoassay. (C) 2020 Elsevier B.V. All rights reserved.
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