Local promotion of B cells (B10) function alleviates experimental periodontitis bone loss through antagonizing Receptor Activator of NF-KappaB Ligand-expressing neutrophils

Background Persistent host immune responses initiated by oral bacteria protect host against infection but may also elicit the process of sustained periodontal inflammation and subsequent alveolar bone loss. Interleukin-10 (IL-10), an anti-inflammatory cytokine, can downregulate pro-inflammatory cytokine and inhibit neutrophil migration in inflammation. IL-10-expressing regulatory B cells (B10) is termed by negatively regulating immune response through IL-10 and are mainly restricted in CD19(+)CD1d(hi)CD5(+)B cells in mice. Our current study was aimed to explore the effect of locally transferred CD19(+)CD1d(hi)CD5(+)B cells on inflammation and alveolar bone loss in an experimental periodontitis mouse model. Methods Ligation plusP.gingivalis(Pg) infection was used to induce periodontitis in a mouse model. CD19(+)CD1d(hi)CD5(+)B cells were sorted by flow cytometry and transferred into the gingivae immediately on the fifth day after ligation. All the mice were sacrificed on day 14 after ligation. Results H&E staining showed that inflammatory cell infiltration was significantly reduced by the CD19(+)CD1d(hi)CD5(+)B cells. Toluidine blue staining showed that the CD19(+)CD1d(hi)CD5(+)B cells alleviated alveolar bone loss in the ligature/Pg-induced periodontitis in mice. Immunohistochemical staining showed Receptor Activator of NF-KappaB Ligand (RANKL), Interleukin-1 beta(IL-1 beta) and Interleukin-17 (IL-17) were decreased after the CD19(+)CD1d(hi)CD5(+)B cell transfer. Immunofluorescent staining showed that IL-10 was increased while the number of Ly6G(+)neutrophil and its RANKL production were decreased in gingival tissue. Conclusions These results indicated that locally transferred CD19(+)CD1d(hi)CD5(+)B cells may alleviate alveolar bone loss through inhibiting pro-inflammatory cytokine expression and RANKL-expressing neutrophils in the periodontitis mouse model.

Automated summary

The study found that locally transferred CD19(+)CD1d(hi)CD5(+)B cells significantly reduced inflammatory cell infiltration and alleviated alveolar bone loss in the experimental periodontitis mouse model by inhibiting pro-inflammatory cytokine expression and RANKL-expressing neutrophils.