期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 84, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2020.108461
关键词
Carob fruit extract-enriched meat; Late-stage T2DM; Functional food; Polyphenols; Insulin signaling pathway; Lipogenesis
资金
- Spanish Project [AGL2014-53207-C2-2-R]
- Santander-UCM Project [PR75/18-21603]
- Spanish Ministry of Education, Culture and Sports [FPU15/02759]
The inclusion of functional bioactive compounds of dietary fiber in meat products has been demonstrated to exert a significant impact on human health. Carob fruit extract (CFE) is a dietary fiber rich in proanthocyanidins with known antioxidant, hypolipidemic and hypoglycemic effects. Consumption of CFE-enriched meat (CFE-RM) may provide interesting benefits in late-stage type 2 diabetes mellitus (T2DM). To explore the antidiabetic mechanisms of CFE-RM, we used a model of late-stage T2DM in Wistar rats fed a high-saturated-fat/high-cholesterol diet (Chol-diet) and injected streptozotocin plus nicotinamide (D group). The effects of CFE-RM were tested by incorporating it into the diet as preventive strategy (ED group) or curative treatment (DE group). CFE-RM had a positive effect on glycemia, enhancing hepatic insulin sensitivity and improving pancreatic beta-cell regeneration in both ED and DE groups. Western blotting and immunohistochemistry suggested that CFE-RM increased levels of insulin receptor beta and phosphatidylinositol-3-kinase, as well as the downstream target phospho-Akt (at Ser(473)). CFE-RM also up-regulated glucose transporter 2, which improves the insulin-mediated glucose uptake by the liver, and promoted phosphorylation of glycogen synthesis kinase-3 beta protein (at ser(9)), consequently increasing the hepatic glycogen content. In addition, CFE-RM decreased fatty liver by suppressing de novo lipogenesis activation due to down-regulation of liver X receptor-alpha/beta, sterol regulatory element binding protein-1c and carbohydrate-response element-binding protein transcription factors. Our findings suggest that the consumption of CFE-RM included in the diet as a functional food should be considered as a suitable nutritional strategy to prevent or manage late-stage T2DM. (C) 2020 Elsevier Inc. All rights reserved.
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