4.5 Article

Deficiency of the serine peptidase Kallikrein 6 does not affect the levels and the pathological accumulation of a-synuclein in mouse brain

期刊

JOURNAL OF NEUROCHEMISTRY
卷 157, 期 6, 页码 2024-2038

出版社

WILEY
DOI: 10.1111/jnc.15199

关键词

alpha-synuclein; clearance; kalikrein 6 peptidase; microdialysis; synucleinopathies; transmission

资金

  1. General Secretariat for Research and Technology [.1EDK Bioluminpd 03884]
  2. Michael J. Fox Foundation for Parkinson's Research

向作者/读者索取更多资源

The roles of KLK6 in regulating alpha-syn processing and pathology transmission in vivo were investigated using Klk6 knock-out mice. The study showed that KLK6 deletion did not impact alpha-syn accumulation or behavior in mice, and active KLK6 did not affect alpha-syn levels in A53T alpha-syn over-expressing mice, indicating that KLK6 alone may not be sufficient to modulate alpha-syn pathology transmission. These findings suggest implications for using recombinant alpha-syn fibrils in studies of alpha-syn turnover.
Several lines of evidence indicate that the propagation of misfolded alpha-synuclein (alpha-syn) plays a central role in the progression and manifestation of Parkinson's disease. Pathogenic alpha-syn species can be present in the extracellular space. Thus, the identification and modulation of the key enzymes implicated in extracellular alpha-syn turnover becomes vital. Kallikrein peptidase 6 has been identified as one of the major alpha-syn degrading enzymes and has been implicated in the clearance of extracellular alpha-syn. However, the physiological role of this enzyme in regulating alpha-syn, in vivo, still remains elusive. Here, by utilizing Klk6 knock-out (Klk6(-/-)) mice as our experimental model, we provide insight into the physiologic relevance of endogenous KLK6 expression on alpha-syn processing. Behavioral phenotyping showed thatKlk6(-/-)mice display no gross behavioral abnormalities. Further in vivo characterization of this mouse model, in the context of alpha-syn accumulation, showed that KLK6 deletion had no impact on the protein levels of intracellular or extracellular alpha-syn. Upon in vivo administration of alpha-syn pre-formed fibrils (PFF), alpha-syn pathologic accumulations were evident both in the brains ofKlk6(-/-)mice and wt mice without significant differences. Intrastriatal delivery of active KLK6, did not affect secreted alpha-syn levels observed in the A53T alpha-syn over-expressing mice. These findings suggest that in the in vivo setting of PFF pathology induction, KLK6 alone is not able to modulate pathology transmission. Our study raises implications for the use of recombinant alpha-syn fibrils in alpha-syn turnover studies.

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