4.7 Article

Quantifying the Monomer-Dimer Equilibrium of Tubulin with Mass Photometry

期刊

JOURNAL OF MOLECULAR BIOLOGY
卷 432, 期 23, 页码 6168-6172

出版社

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2020.10.013

关键词

mass photometry; binding affinity; tubulin; single molecule

资金

  1. ERC [CoG 819593]
  2. Francis Crick Institute
  3. Cancer Research UK [FC001163]
  4. UK Medical Research Council [FC001163]
  5. Wellcome Trust [FC001163]
  6. European Research Council [323042]
  7. Spanish Ministry of Economy, Industry and Competitiveness
  8. Centro de Excelencia Severo Ochoa
  9. CERCA Programme of the Generalitat de Catalunya
  10. European Research Council (ERC) [323042] Funding Source: European Research Council (ERC)

向作者/读者索取更多资源

The alpha beta-tubulin heterodimer is the fundamental building block of microtubules, making it central to several cellular processes. Despite the apparent simplicity of heterodimerisation, the associated energetics and kinetics remain disputed, largely due to experimental challenges associated with quantifying affinities in the 10(-2) s(-1). Our results demonstrate the capabilities of mass photometry for quantifying protein-protein interactions and clarify the energetics and kinetics of tubulin heterodimerisation. (C) 2020 The Author(s). Published by Elsevier Ltd.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据