期刊
JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE
卷 35, 期 17, 页码 3244-3253出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/14767058.2020.1817895
关键词
Adaptive immunity; intra-amniotic inflammation; microbial invasion of the amniotic cavity; preterm delivery; protease; T cells; NK cells
资金
- project PERSONMED Center for the Development of Personalized Medicine in Age-Related Diseases [CZ.02.1.01/0.0/0.0/17_048/0007441]
- Charles University in Prague, Faculty of Medicine in Hradec Kralove, Czech Republic [PROGRES P40/10]
The levels of granzyme A in amniotic fluid are associated with microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI) in pregnancies complicated by preterm prelabor rupture of membranes (PPROM). Women with MIAC have lower levels of granzyme A, while women with sterile IAI have higher levels of granzyme A in their amniotic fluid.
Introduction To determine the levels of granzyme A in amniotic fluid in pregnancies complicated by preterm prelabor rupture of membranes (PPROM), based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). Methods of study A total of 166 women with singleton pregnancies complicated by PPROM were included. Amniocentesis was performed at the time of admission and assessments of MIAC (using both cultivation and non-cultivation techniques) and IAI (interleukin-6 in amniotic fluid) were performed on all subjects. Based on the presence/absence of MIAC and IAI, the women were further divided into the following subgroups: intra-amniotic infection, sterile IAI, colonization, and absence of both MIAC and IAI. Amniotic fluid granzyme A levels were assessed using ELISA. Results Women with MIAC had lower levels of granzyme A in the amniotic fluid than women without this condition (with MIAC: median 15.9 pg/mL vs. without MIAC: median 19.9 pg/mL,p = .03). Women with sterile IAI had higher amniotic fluid granzyme A levels than women with intra-amniotic infection, colonization and women with the absence of either MIAC or IAI (intra-amniotic infection: median 15.6 pg/mL; sterile IAI: median 31.8 pg/mL; colonization: median 16.9 pg/mL; absence of both MIAC and IAI: median 18.8 pg/mL;p = .02). Conclusions The presence of sterile IAI was associated with elevated levels of granzyme A in amniotic fluid.
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