期刊
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
卷 31, 期 10, 页码 -出版社
SPRINGER
DOI: 10.1007/s10856-020-06446-x
关键词
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资金
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [757444]
- Knut and Alice Wallenberg Foundation [WAF 2016.0112]
- Swedish Research Council Formas [216-2014-1247]
- National Council of Science and Technology of Mexico
- Novo Nordisk Foundation [NNF16OC0020792, NNF17CC0027852]
- Marie Curie fellowship programme [746270/H2020-MSCA-IF-2016]
- Uppsala University
In this work, we present a method to fabricate a hyaluronic acid (HA) hydrogel with spatially controlled cell-adhesion properties based on photo-polymerisation cross-linking and functionalization. The approach utilises the same reaction pathway for both steps meaning that it is user-friendly and allows for adaptation at any stage during the fabrication process. Moreover, the process does not require any additional cross-linkers. The hydrogel is formed by UV-initiated radical addition reaction between acrylamide (Am) groups on the HA backbone. Cell adhesion is modulated by functionalising the adhesion peptide sequence arginine-glycine-aspartate onto the hydrogel surface via radical mediated thiol-ene reaction using the non-reacted Am groups. We show that 10 x 10 mu m(2)squares could be patterned with sharp features and a good resolution. The smallest area that could be patterned resulting in good cell adhesion was 25 x 25 mu m(2)squares, showing single-cell adhesion. Mouse brain endothelial cells adhered and remained in culture for up to 7 days on 100 x 100 mu m(2)square patterns. We see potential for this material combination for future use in novel organ-on-chip models and tissue engineering where the location of the cells is of importance and to further study endothelial cell biology.
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