4.7 Article

Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water

期刊

JOURNAL OF HAZARDOUS MATERIALS
卷 405, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124224

关键词

Nano-Al2O3; Antibiotic resistance genes (ARGs); Transduction-like ARG transformation

资金

  1. National Natural Science Foundation of China [81703190, 31470234, 81202163]
  2. China Postdoctoral Science Foundation [45591]
  3. Tianjin Natural Science Foundation [17JCZDJC39100]
  4. Key R&D Program Projects in Shandong Province [2019GSF107019]
  5. Natural Science Foundation of Shandong Province [ZR2017LH058]
  6. Zaozhuang Science and Technology Development Plan Project [2019GX19]
  7. Qingtan Scholar Talent Project of Zaozhuang University [100638]

向作者/读者索取更多资源

This study demonstrates that nano-Al2O3 can serve as a carrier mediating transduction-like ARG transformation, preventing degradation of ARGs and facilitating their transmembrane transport, which is of significant importance for global public health.
The prevalence of various antibiotic resistance genes (ARGs) and resistant bacteria has caused global public health risks. The carrier transport mediated by phages or membrane vesicles is an important way for horizontal transfer of ARGs. Nano metal oxide particles (NMOPs), which can enter cell through the cell membrane, may be used as the carriers of genes. However, whether they can be used as transmembrane delivery vectors for the horizontal ARG transfer remains unknown. Here, we set up a model of MONPs-mediated transfer of ARGs, and demonstrate that NMOPs, especially for nano-Al2O3, can act as carriers mediating the transduction-like ARG transformation in water. The highest transfer rate mediated by nano-Al2O3 is 4.53 x 10(4) cfu/mmol, and it is 10(4) times higher than that of control. Nano-Al2O3 can combine with plasmid coding for ARGs to form high-density package and prevent ARGs from degradation by endonuclease. The results of superresolution fluorescence microscopy and transmission electron microscopy show that nano-Al2O3 can carry ARGs for transmembrane transport. Genome-wide transcription microarray and qPCR indicate that SOS response was closely related to transduction-like ARG transformation mediated by nano-Al2O3. This study is the first to demonstrate that as a new transmembrane carrier, nano-Al2O3 can also cause ARGs diffusion in water.

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