期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 405, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2020.124150
关键词
Epigenetic; DNA methylation; Oxidative stress; Gene expression; Mining
资金
- Natural Science Foundation of Guangdong Province of China [2018A030313875, 2019A1515110750]
- National Natural Science Foundation of China [NSFC-41907299]
- start fund of high level talent researcher for Dongguan University of Technology [GC200109-17]
The study found that the middle and downstream sections of Le'an River were heavily polluted with trace metals, leading to epigenetic effects and oxidative stress in zebrafish.
Epigenetic mechanisms are important for gene expression regulation, which is closely related to human health, and epigenetic effects of polluted water bodies have gained increasing research attention. Le'an River suffers from severe trace metal pollution owing to mining activities. In this study, zebrafish was used as a biological model to study pollution of Le'an River after seven consecutive days of exposure. The results showed that midstream and downstream sections of the river were seriously polluted by trace metals. The liver and gill of zebrafish were enriched with trace metals, and cadmium had the highest bioaccumulation factor. Trace metals caused oxidative stress in zebrafish cells, with increases in reactive oxygen species levels. Significant increase of global DNA methylation in liver of middle and downstream section were observed, with values from 125.67% to 165.45% compared with control. Changes in DNA methylation in the promoter region cause significant increase or decrease of the expression of repair genes and apoptosis genes in liver and gill. In summary, Le' an River water exhibited significant epigenetic effects, and it is necessary to consider epigenetic effects in the evaluation of pollution and health risks of river water.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据