Investigation of the bacterial cell envelope nanomechanical properties after long-term exposure to nitrofurans

Antibiotic residues in the environment may negatively affect biological communities in the natural ecosystems. However, their influence on environmental bacterial strains has not been thoroughly investigated. In this study, two representatives of 5-nitrofuran antibiotics (nitrofurantoin and furaltadone) were investigated in terms of their long-term influence on the cell envelopes of newly isolated environmental bacterial strains (Sphingo-bacterium caeni FTD2, Achromobacter xylosoxidans NFZ2 and Pseudomonas hibiscicola FZD2). A 12-month exposure of bacterial cells to nitrofurans at a concentration of 20 mg L-1 induced changes in the cell structure and texture (bacteria under stress conditions showed a loss of their original shape and seemed to be vastly inflated, the cells increased average surface roughness after exposure to NFT and FTD, respectively). AFM observations allowed the calculation of the bacterial cell nanomechanical properties. Significant increase in adhesion energy of bacteria after prolonged contact with nitrofurantoin was demonstrated. Changes in the permeability of bacterial membrane, fatty acids' composition and bacterial cell surface hydrophobicity were determined. Despite visible bacterial adaptation to nitrofurans, prolonged presence of pharmaceuticals in the environment has led to significant alterations in the cells' structures which was particularly visible in P. hibiscicola.

Automated summary

The study found that antibiotic residues in the environment have long-term effects on the cell envelopes of newly isolated environmental bacterial strains, leading to changes in cell structure, texture, and adhesion energy. Bacteria showed some adaptation to nitrofurans, but prolonged exposure to these pharmaceuticals resulted in significant alterations in cell structures, especially in P. hibiscicola.