4.5 Article

Rabbit umbilical cord mesenchymal stem cells: A new option for tissue engineering

期刊

JOURNAL OF GENE MEDICINE
卷 23, 期 1, 页码 -

出版社

WILEY
DOI: 10.1002/jgm.3282

关键词

heterotransplantation; immunological characteristics; rabbit umbilical cord mesenchymal stem cells; regeneration medicine; tissue engineering

资金

  1. National Natural Science Foundation of China (NSFC) [81670951, 81800962]

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The research on rabbit umbilical cord mesenchymal stem cells showed that these cells have high proliferation and multi-lineage differentiation potential, as well as excellent immunogenicity and immune suppression properties. In vivo studies demonstrated that rabbit umbilical cord mesenchymal stem cells can promote osteogenesis in heterogeneous hosts.
Background: The source and availability of cells for tissue engineering in large scale research or clinical trials requires special attention. We propose the idea of applying rabbit umbilical cord mesenchymal stem cells for this purpose. Methods: Here, the structure of the rabbit umbilical cord was analyzed and compared to that of human umbilical cord, both macroscopically and histologically. Next, we isolated, cultured and identified the proliferative activity and immunological characteristics of rabbit umbilical cord mesenchymal stem cells in vitro using mixed lymphocyte reaction, flow cytometry and an enzyme-linked immunosorbent assay. Furthermore, we evaluated the effects of biphasic calcium phosphate ceramic scaffolds seeded with rabbit umbilical cord mesenchymal stem cells in rat cranial defect models using multiple techniques, including radiological, histological and immunohistochemistry. Results: In vitro studies demonstated a high level of proliferation and multi-lineage differentiation potential in rabbit umbilical cord mesenchymal stem cells. Rabbit umbilical cord mesenchymal stem cells exibited low immunogenicity properties and immune suppression capability with respect to both the allogeneic and xenogeneic immune response. The results of the in vivo study showed that rabbit umbilical cord mesenchymal stem cells could promote osteogenesis in heterogeneous hosts. Conclusions: The rabbit umbilical cord mesenchymal stem cells may be a new source for tissue engineering.

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