期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 72, 期 4, 页码 1212-1224出版社
OXFORD UNIV PRESS
DOI: 10.1093/jxb/eraa497
关键词
Carotenoids; photosynthesis; plant architecture; rice; strigolactones; Z-ISO
资金
- National Natural Science Foundation of China [91335202, 91535301]
- Chinese Universities Fund [2020TC162]
HTD12 gene plays a crucial role in rice, regulating chloroplast development, photosynthesis, and plant architecture by controlling carotenoid biosynthesis and affecting strigolactone concentrations.
Photosynthesis and plant architecture are important factors influencing grain yield in rice (Oryza sativa L.). Here, we identified a high-tillering and dwarf 12 (htd12) mutant and analyzed the effects of the HTD12 mutation on these important factors. HTD12 encodes a 15-cis-zeta-carotene isomerase (Z-ISO) belonging to the nitrite and nitric oxide reductase U (NnrU) protein family, as revealed by positional mapping and transformation experiments. Sequence analysis showed that a single nucleotide transition from guanine (G) to adenine (A) in the 3' acceptor site between the first intron and second exon of HTD12 alters its mRNA splicing in htd12 plants, resulting in a 49-amino acid deletion that affects carotenoid biosynthesis and photosynthesis. In addition, compared with the wild type, htd12 had significantly lower concentrations of ent-2'-epi-5-deoxystrigol (epi-5DS), a native strigolactone, in both roots and root exudates, resulting in an obvious increase in tiller number and decrease in plant height. These findings indicate that HTD12, the rice homolog of Z-ISO, regulates chloroplast development and photosynthesis by functioning in carotenoid biosynthesis, and modulates plant architecture by affecting strigolactone concentrations.
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