Fate and transfer of heavy metals following repeated biogas slurry application in a rice-wheat crop rotation

The application of biogas slurry, from anaerobic digestion of livestock excreta, to cropland has proven to be an effective mechanism for recycling nutrients within farms. However, the potential pollution of heavy metals from repeated biogas slurry fertilization has not received much attention. Here we present the results of a field experiment under rice-wheat rotation demonstrating the accumulation, speciation distribution and plant uptake of heavy metals (Cu, Zn, Pb and Cd) in soil following biogas slurry application. The treatments were: zero biogas slurry application (BS0), and biogas slurry application for three (BS3) and five (BS5) years, at a rate of 450 m(3) ha(-1) y(-1). Our findings show that biogas slurry fertilization resulted in accumulation of Cu and Zn in the soil. The concentrations of soil Cu and Zn under BS5 were, respectively, 38 and 29% greater in the wheat season and 35 and 35% greater in the rice season relative to BS0 (p < 0.05). The bioavailability of soil Cu and Zn increased following biogas slurry application. Plant uptake of Cu and Zn to all parts of wheat and rice plants (excluding Cu in wheat straw) increased with the years of biogas slurry application (p < 0.05), and the concentration of Cd in wheat grain was significantly greater in BS5 relative to BS0 (p < 0.05). After five years of biogas slurry fertilization, concentrations of Cu, Zn, Pb and Cd in wheat grains were 3.28, 25.19, 0.11 and 0.053 mg kg(-1) and 4.24, 33.78, 0.12 and 0.035 mg kg(-1) for rice grains, respectively, all within the safety limits. Our results demonstrate that repeated biogas slurry fertilization for five years has a relatively low pollution risk of heavy metals. However, long-term field monitoring and co-application with metal-immobilizing materials are required to ensure the safety of its application to cropland.