期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 576, 期 -, 页码 322-329出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2020.05.026
关键词
3 dimensional; Rotational diffusion; Colloidal spheres; Single particle tracking; Raspberry colloids; Rotational probes; Confocal scanning laser microscopy
资金
- NWO-CW (ECHO) [712.016.004]
Real time visualization and tracking of colloidal particles with 3D resolution is essential for probing the local structure and dynamics in complex fluids. Although tracking translational motion of spherical particles is well-known, accessing rotational dynamics of such particles remains a great challenge. Here, we report a novel approach of using fluorescently labeled raspberry-like colloids with an optical anisotropy to concurrently track translational and rotational dynamics in 3 dimensions. The raspberry-like particles are coated by a silica layer of adjustable thickness, which allows tuning the surface roughness. The synthesis and applicability of the proposed method is demonstrated by two types of probes: rough and smoothened. The accuracies of measuring Mean Squared (Angular) Displacements are also demonstrated by using these 2 probes dispersed in 2 different solvents. The presented 3D trackable colloids offer a high potential for wide range of applications and studies, such as probing the dynamics of crystallization, phase transitions, biological interactions and the effect of surface roughness on diffusion. (C) 2020 The Authors. Published by Elsevier Inc.
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