期刊
JOURNAL OF CLINICAL PERIODONTOLOGY
卷 48, 期 1, 页码 76-90出版社
WILEY
DOI: 10.1111/jcpe.13389
关键词
cytokines; periodontal debridement; periodontitis; T lymphocytes; helper-induced; T lymphocytes; regulatory
资金
- National Health and Medical Research Council [APP1029878]
- Australian Dental Research Foundation (ADRF) [211-2017]
This study evaluated the longitudinal variation in peripheral T helper cells in periodontitis patients undergoing management, indicating potential roles of IFN-gamma(+) and Foxp3(+) cells in the systemic compartment of periodontitis. Treatment outcomes were associated with changes in the expression of IFN-gamma and Foxp3 cells. Periodontal management has local and systemic effects, highlighting the importance of assessing and managing periodontitis for overall systemic health.
Aim: Periodontitis has been associated with other systemic diseases with underlying inflammation responsible for the shared link. This study evaluated longitudinal variation in peripheral T helper cells in periodontitis patients undergoing management over 1 year. Materials and methods: Periodontal parameters and peripheral blood mononuclear cells (PBMCs) were collected from 54 periodontitis patients at baseline, and 3-, 6- and 12-months post-treatment and 40 healthy controls. IFN-gamma(+), IL-4(+), IL-17(+) and Foxp3(+) and their double-positive expression were identified in CD4(+) and TCR alpha beta(+) cells using flow cytometry. PBMCs were incubated with P. gingivalis, and IFN-gamma, IL-4, IL-17 and IL-10 in cell supernatant were measured by ELISA. Cells and cytokines were also assessed based on clinical response to treatment where good (<10% of sites), moderate (10-20%) and poor (>20%) treatment outcome (TxO) groups had probing depths of >= 5 mm at study conclusion. Results: IFN-gamma(+) cells were lower at baseline, and 3- and 6-months compared to health, whereas Foxp3(+) cells were increased at 12-months compared to all preceding timepoints and health. The good TxO group showed treatment-related variation in IFN-gamma(+) and Foxp3(+) cells, whereas the poor TxO group did not. IFN-gamma and IL-17 cytokine expression in cell supernatants was significantly lower at baseline compared to health, and IFN-gamma and IL-10 showed treatment-related decrease. Conclusion: This study suggests that IFN-gamma(+) and Foxp3(+) cells may have a role in the systemic compartment in periodontitis. Periodontal management has local and systemic effects, and thus, assessment and management of periodontitis should form an integral part of overall systemic health.
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