期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 59, 期 1, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01764-20
关键词
group A Streptococcus; M protein; vaccine; emm typing; multiplex real-time PCR
类别
The study developed a real-time PCR-based method for rapid identification of emm types in A streptococcus, showing high specificity and accurately identifying target emm types, useful for rapid typing of bacterial isolates and culture-negative specimens during outbreak investigations.
We developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention's Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.
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