期刊
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
卷 32, 期 2, 页码 229-247出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2020.1827922
关键词
Biodegradable; microsphere; osteoconductive; osteoinductive; bone regeneration
资金
- National Key R&D Program of China [2017YFC1104300/4302]
- National Natural Science Foundation of China [51873018]
- Beijing Municipal Natural Science Foundation [7182068, 7161001]
This study prepared microspheres with surface micropores using the double emulsion method, which showed increased cell affinity and enhanced osteogenic differentiation of BMSCs after further functionalization. By adsorbing BMP-2 onto the biomineralized microspheres, efficient ectopic osteogenesis was induced without causing biocompatibility problems. The study provided a simple strategy to prepare functionalized microspheres with great potential in promoting bone regeneration as injectable micro-scaffolds.
There are intensive needs for scaffolds with new designs to meet the diverse requirements of bone repairing. Biodegradable microspheres are highlighted as injectable micro-scaffolds thanks to their advantages in filling irregular defects via a minimally invasive surgery. In this study, microspheres with surface micropores were made via the W1/O/W2 double emulsion method using amphiphilic triblock copolymers (PLLA-PEG-PLLA) composed of poly(L-lactide) (PLLA) and poly(ethylene glycol) (PEG) segments. When the PEG fraction was controlled as 10wt.%, the microspheres demonstrated higher cell affinity than the smooth-surfaced PLLA microspheres. After being further functionalized with polydopamine coating and apatite deposition, the PLLA-PEG-PLLA microspheres could up-regulate the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSCs) significantly. Before subcutaneous implantation, bone morphogenetic protein-2 (BMP-2) was adsorbed onto the biomineralized microspheres by taking advantages of the strong affinity of apatite to BMP-2. The resulted microspheres induced ectopic osteogenesis efficiently without causing biocompatibility problems. In summary, this study provided a simple strategy to prepare functionalized microspheres with osteoconductivity and osteoinductivity, which showed great potential in promoting bone regeneration as injectable micro-scaffolds.
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