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Standard methods for Apis mellifera venom research

期刊

JOURNAL OF APICULTURAL RESEARCH
卷 60, 期 4, 页码 -

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/00218839.2020.1801073

关键词

acid phosphatase; Apis mellifera; esterase; honey bee; hyaluronidase; phospholipase A2; sting allergy; venom characterization; venom collection; venom composition; venom storage; COLOSS; BEEBOOK

资金

  1. COST Action [FA0803]
  2. EU Seventh Framework Program for research, technological development, and demonstration activities
  3. Ricola Foundation -Nature Culture
  4. EC Grant Agreement

向作者/读者索取更多资源

Honey bees possess a sting used to inject venom, sparking interest in its potential therapeutic and allergenic properties. This paper provides insight into standardized methods for researching honey bee venom, covering collection techniques, biological activity determination, and application in biomedical research.
Honey bees have a sting which allows them to inject venomous substances into the body of an opponent or attacker. As the sting originates from a modified ovipositor, it only occurs in the female insect, and this is a defining feature of the bee species that belong to a subclade of the Hymenoptera called Aculeata. There is considerable interest in bee venom research, primarily because of an important subset of the human population who will develop a sometimes life threatening allergic response after a bee sting. However, the use of honey bee venom goes much further, with alleged healing properties in ancient therapies and recent research. The present paper aims to standardize selected methods for honey bee venom research. It covers different methods of venom collection, characterization and storage. Much attention was also addressed to the determination of the biological activity of the venom and its use in the context of biomedical research, more specifically venom allergy. Finally, the procedure for the assignment of new venom allergens has been presented.

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