The effects of eggshell calcium (Biomin H(R)) and its combinations with alfacalcidol (1α-hydroxyvitamin D3) and menaquinone-7 (vitamin K2) on ovariectomy-induced bone loss in a rat model of osteoporosis

The purpose of this study was to investigate the impact of eggshell calcium (Biomin H(R)dietary supplement) and its combinations with alfacalcidol (1 alpha-hydroxyvitamin D-3) and menaquinone-7 (vitamin K-2) on ovariectomy-induced bone loss in rats. Adult female rats (n = 48) were divided into 6 groups of 8 individuals each: sham-operated rats (SHAM); ovariectomized (OVX) rats untreated; OVX rats treated with Biomin H(R)(BIO); OVX rats simultaneously receiving Biomin H(R), vitamin D-3(BIO + D-3); OVX rats simultaneously treated with Biomin H(R), vitamin K-2(BIO + K-2) and OVX rats treated with Biomin H(R), vitamin D-3, vitamin K-2(BIO + D-3 + K-2) during 8 weeks. Biochemical parameters, bone mineral density (BMD), bone mineral content (BMC) and femoral bone microstructure were determined. Plasma calcium and phosphate were increased in BIO + D(3)and BIO + D-3 + K-2 groups as compared to OVX. Alkaline phosphatase was elevated in OVX, BIO versus SHAM, BIO + D-3 + K2 groups. When compared to OVX group, decreased urine deoxypyridinoline was observed in all treated groups and femoral BMD, BMC were higher in BIO, BIO + D-3, BIO + D-3 + K-2 groups. The BIO + K-2 rats had similar densitometrical values than OVX individuals. Microcomputed tomography revealed increased trabecular relative bone volume (due to an increase in trabecular number) in BIO + D-3, BIO + D-3 + K-2 as compared to OVX. The higher relative bone volume in BIO + D-3, BIO + D-3 + K(2)groups was also accompanied by an increase in bone surface. In the cortical bone, an enhanced periosteal bone apposition was identified in BIO, BIO + D-3, BIO + K-2, BIO + D-3 + K-2 groups. The rats from BIO + D-3 + K-2 group had a higher area of primary osteon's vascular canals. In BIO + D-3, BIO + K-2, BIO + D-3 + K-2 groups, an increased area of secondary osteons was determined in comparison with OVX. Our results indicate the beneficial effect of triple application of Biomin H(R), vitamin D-3, vitamin K-2, as well as simultaneous administration of Biomin H(R), vitamin D(3)on the inhibition of ovariectomy-induced bone loss in a rat model of osteoporosis.

Automated summary

This study investigated the impact of eggshell calcium (Biomin H(R) dietary supplement) and its combinations with alfacalcidol (1 alpha-hydroxyvitamin D-3) and menaquinone-7 (vitamin K-2) on ovariectomy-induced bone loss in rats. Results showed the beneficial effect of triple application of Biomin H(R), vitamin D-3, vitamin K-2 on the inhibition of ovariectomy-induced bone loss, with significant improvements in femoral bone mineral density and bone mineral content observed in treated groups.