期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 68, 期 42, 页码 11702-11709出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c02476
关键词
lupin; peptide; anti-inflammation; RNA-sequencing analysis
资金
- National Natural Science Foundation of China [31701597]
- Central Public-interest Scientific Institution Basal Research Fund [Y2020PT30]
- NCGRC. national crop germplasm resource center [NCGRC-2020-8]
- China Agriculture Research System [CARS-08-G20]
- Agricultural Science and Technology Innovation Program of CAAS [CAAS-ASTIP-2017ICS]
In this study, protein was extracted from extruded lupin and submitted to gastroduodenal digests to obtain lupin peptides, which were characterized using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After this, IQDKEGIPPDQQR (IQD), the lupine peptide monomer characterized after UPLC-MS/MS, was screened out by macrophage inflammatory cytokine production assay. RNA-sequencing analysis was performed to explore the mechanisms underlying the anti-inflammatory activity associated with this peptide. The results indicated that lupin peptides effectively inhibited the lipopolysaccharide-induced overproduction of proinflammatory mediators. IQD inhibited the production of tumor necrosis factor-alpha, interleukin (IL)-6, IL-1 beta, and monocyte chemoattractant protein-1 by 51.20, 38.52, 44.70, and 40.43%, respectively. RNA-sequencing results showed that IQD inhibited the inflammatory response by regulating the gene expression of the p38 mitogen-activated protein kinase pathway and inhibiting downstream inflammatory cytokines. These bioactive peptides may be used to develop new ingredients for anti-inflammatory nutritional supplements.
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