期刊
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
卷 27, 期 1, 页码 627-639出版社
SPRINGER
DOI: 10.1007/s10989-020-10113-8
关键词
Alzheimer's disease; BACE1; Oxidative stress; Amyloid-beta; hydrolysate
资金
- Ministry of Oceans and Fisheries (MOF) [20140441]
The study found that peptides in the olive flounder skin hydrolysates can effectively inhibit beta-secretase activity, potentially serving as ingredients for new pharmaceutical and nutraceutical agents for the treatment of Alzheimer's Disease.
The prevalence of degenerative neuro-diseases such as Alzheimer's disease has increased with an increase in the life expectancy especially as seen in developed countries. According to the Amyloid Hypothesis, beta-secretase leads to the onset of Alzheimer's disease though the generation of amyloid-beta by proteolysis of amyloid precursor protein. Amyloid-beta aggregates to form oligomers that build up to form plaques. Olive flounder (Paralichthys olivaceus) is high in protein content, with a higher protein content in skin (27.65%) compared to muscle (19.65%) according to proximate analysis results. Enzyme hydrolysates were prepared from skin and muscle using several enzymes. The neutrase skin hydrolysate showed the highest beta-secretase inhibitory activity IC50 0.61 mg/mL. Purification steps were performed using ion exchange chromatography on QMA and CM-cellulose columns and SPE C18 column to produce an active fraction QMA-F1 with an IC50 value of 32.80 mu g/mL. QMA-F1 was non-cytotoxic to SH-SY5Y cells, reduced BACE1 overexpression and attenuated amyloidogenesis. This suggests that the active beta-secretase inhibitory peptides from olive flounder skin hydrolysates can be utilized as an ingredient in development of new pharmaceutical and nutraceutical therapeutic agents for Alzheimer's Disease.
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