期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms21176338
关键词
bacteriophage; phage therapy; Stenotrophomonas; phage genomics; phage receptor
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- CGS-D award from NSERC
- AIGSS award from Alberta Innovates
- NSERC USRA summer studentship
The rapid increase in the number of worldwide human infections caused by the extremely antibiotic resistant bacterial pathogenStenotrophomonas maltophiliais cause for concern. An alternative treatment solution in the post-antibiotic era is phage therapy, the use of bacteriophages to selectively kill bacterial pathogens. In this study, the novel bacteriophage AXL3 (vB_SmaS-AXL_3) was isolated from soil and characterized. Host range analysis using a panel of 29 clinicalS. maltophiliaisolates shows successful infection of five isolates and electron microscopy indicates that AXL3 is a member of theSiphoviridaefamily. Complete genome sequencing and analysis reveals a 47.5 kb genome predicted to encode 65 proteins. Functionality testing suggests AXL3 is a virulent phage and results show that AXL3 uses the type IV pilus, a virulence factor on the cell surface, as its receptor across its host range. This research identifies a novel virulent phage and characterization suggests that AXL3 is a promising phage therapy candidate, with future research examining modification through genetic engineering to broaden its host range.
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