期刊
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
卷 135, 期 1, 页码 81-89出版社
SPRINGER
DOI: 10.1007/s00414-020-02398-7
关键词
Forensic science; Ancestry markers; External visible characteristics; Massively parallel DNA sequencing; STR DNA profiling; Trace DNA
资金
- Defence Science and Technology Group (DST Group) of the Australia Department of Defence and Combating Terrorism Technical Support Office (CTTSO) of the US Department of Defence
- Attorney General's Department through Forensic Science SA
- Ross Vining Memorial Research Fund
- DPST scholarship
This study presents the first application of direct PCR coupled with MPS to generate forensic intelligence SNP data from latent DNA. By analysing results from 60 touched samples, the study demonstrated the ability of this approach to obtain informative genetic data from trace amounts of DNA.
Direct PCR can be used to successfully generate full STR profiles from DNA present on the surface of objects. STR profiles are only of use in cases where a potential donor profile is available for comparison, and DNA is of sufficient DNA quality and quantity to generate a reliable profile. Often, no donor information is available and only trace DNA is present on items. As a result, alternative techniques are required to generate genetic data that can provide investigative leads. Massively parallel sequencing (MPS) offers the ability to detect trace levels of DNA and improve DNA analysis success from touched items. Here, we present the first application of direct PCR coupled with MPS to generate forensic intelligence SNP data from latent DNA. The panels assessed are (1) the HIrisplex System that targets 24 SNPs to simultaneously predict hair and eye, and (2) the Precision ID Ancestry Panel that targets 165 autosomal SNPs indicative of biogeographic ancestry. For each panel, we analysed 60 touched samples across five individuals and four substrates (glass slide, fuse, zip-lock bag and wire) using Ion AmpliSeq Library Preparation Kit on the automated Ion Chef System and Ion Torrent PGM. We examine the SNP recovery, concordance with reference samples and the genotype reproducibility from different substrates and donors. The results demonstrate the application of this approach for obtaining informative genetic from trace amounts of DNA.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据