4.7 Article

A heterologously-expressed thermostable Pyrococcus furiosus cytoplasmic [NiFe]-hydrogenase I used as the catalyst of H2/air biofuel cells

期刊

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
卷 46, 期 4, 页码 3035-3044

出版社

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ijhydene.2020.08.155

关键词

Enzymatic H-2/air biofuel cell; Hydrogenase; Heterologous expression; High-potential deactivation; O-2 tolerance

资金

  1. National Natural Science Foundation of China [21878324]
  2. Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [TSBICIP-KJGG-004-001]
  3. CAS Pioneer Hundred Talent Program (Type C) [2016-081]

向作者/读者索取更多资源

A thermophilic archaea host system was developed to express a thermostable Pyrococcus furiosus cytoplasmic [NiFe]-hydrogenase I, which showed high activity and stability at elevated temperatures. The recombinant hydrogenase could be a good candidate for catalyzing H-2/air biofuel cells, with promising electrochemical performance and oxygen tolerance.
Hydrogenase-catalyzed H-2/air biofuel cells have attracted wide attention. However, how to obtain thermostable and highly active hydrogenase remains challenging. Herein, we developed a thermophilic archaea host system to heterologously express a thermostable Pyrococcus furiosus cytoplasmic [NiFe]-hydrogenase I (PfSHI). The recombinant hydrogenase could be easily isolated and then immobilized onto a carbon nanotube-modified carbon felt electrode for further electrochemical characterization. The bioanode was able to work at a wide range of temperatures from 40 degrees C to 80 degrees C and no high potential deactivation was observed until 0.3 V at 50 degrees C. Cyclic voltammograms at elevated temperatures for reverse H-2 oxidation performance indicated that an inactive state might be formed and then reversed when scanning to negative position. Also, the power density of a whole cell reached 1.08 mW cm(-2) using Pt/C as the cathode with a brilliant open circuit potential reaching 1.2 V. The voltage could retain 90% of its initial value after 33 h discharge at a current of 10 mu A. Besides, the bioanode exhibited decent oxygen tolerance both in ambient and elevated temperatures. These results suggest that this recombinant PfSHI can be a good candidate for catalyzing H-2/air biofuel cells. (c) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据