期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 161, 期 -, 页码 1181-1188出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.06.140
关键词
Bacillus megaterium; Levan; Purification; Characterization; Antioxidant activity
资金
- Foundation of Heilongjiang Educational Committee [2017-KYYWF-0699, 2017-KYYWF-0695]
An exopolysaccharide (EPS)-producing bacterial PFY-147 was separated from Hiqiher vineyard soil and determined as Bacillus megaterium by 16S rDNA analysis and morphological. The PFY-147-EPS was purified by gel-filtration chromatography. The average molecular weight (Mw) of purified EPS was 1.878 x 10(6) Da. The total carbohydrate contents, uronic acid and sulfated group content in PFY-147-EPS were found to be 90.37 +/- 1.48%, 7.24 +/- 0.36% and 2.39 +/- 0.36%, respectively. The PFY-147-EPS was found to be a levan containing a backbone of 2,6-substituted beta-fructoses by high performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) and Fourier transform infrared (FT-IR) spectroscopy. The atomic force microscopy (AFM) presented that the levan formed pointed or thorny structural in aqueous solution. Scanning electron microscopy (SEM) of the levan showed compact and nonuniform block sizes morphology. Moreover, the levan exhibited higher thermal stability with a degradation temperature of 265.63 degrees C in DSC analysis. The levan possessed strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, Superoxide anion (O2-) radical, hydroxyl (center dot OH) radical and and2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(+)) scavenging activities and strong metal ion chelating activity. The WHC and WSI of levan were 231.29 +/- 3.76% and 97.34 +/- 1.72%, respectively. These might have implications in food and medicine, as well as in pharmaceutical industries. (C) 2020 Elsevier B.V. All rights reserved.
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