期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 159, 期 -, 页码 415-421出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.05.012
关键词
Sulfated polysaccharide; Chemical structure; Antithrombotic and antiplatelet activities
资金
- National Council for Scientific and Technological Development (CNPq)
- Council for Advanced Professional Training (CAPES)
- Research Council of the State of Ceara(Funcap)
A sulfated polysaccharide from the red algae Gelidiella acerosa (GaSP) was obtained through enzymatic extraction and subjected to chemical characterization by HPSEC, elemental microanalysis, FT-IR and NMR spectroscopies. The GaSP anticoagulant activity was investigated through APTT and PT tests and platelet aggregation assessed by turbidimetry. The antithrombotic and hemorrhagic activities were evaluated by venous thrombosis and hemorrhagic tendency models, respectively. FT-IR and NMR demonstrated that GaSP is a sulfated agaran. HPSEC and elemental microanalysis revealed a peak molar mass of 284.8 kDa and a degree of sulfation of 0.63, respectively. This molecule prolonged the coagulation time in 2.1 times and inhibited the platelet aggregation by 45%. Furthermore, it showed significant dose-dependent antithrombotic effect of 40%, 64% and 80% at 0.1, 0.5 and 1 mg/kg, respectively, without hemorrhage. These results suggest that GaSP has promising antithrombotic. (c) 2020 Elsevier B.V. All rights reserved.
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