期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 165, 期 -, 页码 2765-2772出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.10.154
关键词
Osteoarthritis; Hyaluronic acid; Antioxidant
资金
- Ministry of Science and Technology [107-2314-B-010-010, 108-2314-B-010-042-MY3, 108-2320-B-006-053]
- Taipei Veterans General Hospital [V108C-152, V109C-072]
- Center For Intelligent Drug Systems and Smart Bio-devices (IDS2B) fromThe Featured Areas Research Center Program
- Higher Education Sprout Project, Ministry of Education
Hyaluronic acid (HA) injection into the osteoarthritis (OA) knee is one of the most popular treatment methods. The study aimed to determine whether HA exhibits antioxidant and antiapoptotic functions in the treatment of OA. Sixty-two outpatient patients with a diagnosis of knee OA were recruited. All patients received (HA) injections twice at a 2-week interval. Synovial fluid through sono-guided aspiration was collected for neutrophils isolation. Oxidative stress, apoptotic markers and related pathways in neutrophils were investigated. Among the oxidative stress markers, 4-hydroxynonenal (4-HNE) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) significantly decreased after HA injection, while superoxide dismutase (SOD) and catalase did not change, which indicated that HA injection had an antioxidant effect that was not through activation of antioxidant enzymes. In addition, we found that HA injection decreased p-AKT levels and decreased p-p53 and p-p38 but not p-GSK-3 beta. Moreover, we confirmed that HA injection reduced proapoptotic markers through amitochondria-dependent pathway and proinflammatory events. In vitro investigations also confirmed that HA reduced TNF-alpha-caused apoptosis in chondrocytes, however, this phenomenon was vanished by AKT inhibitor. Taken together, HA injection into human OA knees resulted antioxidant and antiapoptotic functions, as well as reduced inflammation, through modulation of the AKT pathway. (C) 2020 Elsevier B.V. All rights reserved.
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