Epidermal growth factor receptor conjugated fucoidan/alginates loaded hydrogel for activating EGFR/AKT signaling pathways in colon cancer cells during targeted photodynamic therapy

In the present study, we developed epidermal growth factor receptor conjugated fucoidan/alginate loaded hydrogels for targeting the delivery of hydrogel through the signaling pathway of the epidermal growth factor receptor (EGFR) to treat colon cancer. We aim to develop a drug delivery system of chlorin e6 encapsulated in hydrogel and tag it with EFGR to target cancer cells with low toxicity and limited side effects by using photodynamic therapy (PDT). The characterization and in vitro studies were conducted to evaluate the efficiency of the EGFR-hydrogel in colon cancer cells. Also, western blot analysis was used to assess protein expression levels. The in vitro results confirmed significant cell viability, proliferation, and migration of hydrogel in colon cancer. The cellular effects of the EFGR/AKT pathway were cell proliferation, inhibition of apoptosis, cell cycle progression, and cell survival and migration of colon cancer because of significant protein expression levels. The data suggested that hydrogel appears to be a promising targeting approach-PDT for treating colon cancer. Further in vivo studies are needed to conclude the overexpression level of EGFR on cancer cells. The study concluded that EGFRH improved the targeting efficiency of hydrogel in colon cancer. (C) 2020 Elsevier B.V. All rights reserved.