期刊
IMMUNITY
卷 53, 期 5, 页码 1001-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2020.09.003
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资金
- NIH [R01DK093674, R01DK113375, R21A144827]
- Mathers Foundation
- Pershing Square Foundation
- Black Family Metastasis Center
- FASI/FARE Consortium
The gut epithelium is populated by intraepithelial lymphocytes (IELs), a heterogeneous T cell population with cytotoxic and regulatory properties, which can be acquired at the epithelial layer. However, the role of T cell receptor (TCR) in this process remains unclear. Single-cell transcriptomic analyses revealed distinct clonal expansions between cell states, with CD4 (+)CD8 alpha alpha (+) IELs being one of the least diverse populations. Conditional deletion of TCR on differentiating CD4 (+) T cells or of major histocompatibility complex (MHC) class II on intestinal epithelial cells prevented CD4 (+)CD8 alpha alpha (+) IEL differentiation. However, TCR ablation on differentiated CD4 (+)CD8 alpha alpha (+) IELs or long-term cognate antigen withdraw did not affect their maintenance. TCR re-engagement of antigen-specific CD4 (+)CD8 alpha alpha (+) IELs by Listeria monocytogenes did not alter their state but correlated with reduced bacterial invasion. Thus, local antigen recognition is an essential signal for differentiation of CD4 (+) T cells at the epithelium, yet differentiated IELs are able to preserve an effector program in the absence of TCR signaling.
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