期刊
GYNECOLOGIC ONCOLOGY
卷 160, 期 1, 页码 161-168出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygyno.2020.10.012
关键词
Endometrial; Neoplasm; Cancer; Mismatch repair; Lynch syndrome; Somatic; Double somatic
资金
- Pelotonia, United States of America
- Cancer Center Support Grant at the Ohio State University Comprehensive Cancer Center from the National Cancer Institute, United States of America [P30 CA016058]
The study revealed that double somatic MMR gene PV are as common as Lynch syndrome among endometrial cancer patients. Paired tumor and germline testing for patients with non-methylated dMMR tumor may be the most efficient approach for Lynch syndrome screening.
Objective. Lynch syndrome is the most common cause of inherited endometrial cancer, attributable to germline pathogenic variants (PV) in mismatch repair (MMR) genes. Tumor microsatellite instability (MSI-high) and MMR IHC abnormalities are characteristics of Lynch syndrome. Double somatic MMR gene PV also cause MSI-high endometrial cancers. The aim of this study was to determine the relative frequency of Lynch syndrome and double somatic MMR PV. Methods. 341 endometrial cancer patients enrolled in the Ohio Colorectal Cancer Prevention initiative at The Ohio State University Comprehensive Cancer Center from 1/1/13-12/31/16. All tumors underwent immunohistochemical (IHC) staining for the four MMR proteins, MSI testing, and MUD methylation testing if the tumor was MMR-deficient (dMMR). Germline genetic testing for Lynch syndrome was undertaken for all cases with dMMR tumors lacking MLH1 methylation. Tumor sequencing followed if a germline MMR gene PV was not identified. Results. Twenty-seven percent (91/341) of tumors were either MSI-high or had abnormal IHC indicating dMMR. As expected, most dMMR tumors had MLH1 methylation; (69, 75.8% of the dMMR cases; 20.2% of total). Among the 22 (6.5%) cases with dMMR not explained by methylation, 10 (2.9% of total) were found to have Lynch syndrome (6 MSH6, 3 MSH2, 1 PMS2). Double somatic MMR PV accounted for the remaining 12 dMMR cases (3.5% of total). Conclusions. Since double somatic MMR gene PV are as common as Lynch syndrome among endometrial cancer patients, paired tumor and germline testing for patients with non-methylated dMMR tumor may be the most efficient approach for LS screening. (C) 2020 Elsevier Inc. All rights reserved.
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