期刊
GENETICS
卷 216, 期 2, 页码 481-497出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/genetics.120.303549
关键词
secondary metabolites; specialized metabolism; gliotoxin; chemodiversity; pathogenicity
资金
- Howard Hughes Medical Institute through the James H. Gilliam Fellowships for Advanced Study program
- Vanderbilt University
- National Science Foundation [DEB-1442113]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2016/07870-9]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- National Cancer Institute [P01 CA125066]
- National Institutes of Health via the National Center for Complementary and Integrative Health [F31 AT010558]
- National Institute of General Medical Sciences [T34 GM113860]
Aspergillus fumigatusis a major fungal pathogen of humans but its two closest relatives,Aspergillus fischeriandAspergillus oerlinghausenensis, are not. Steenwyket al.examined whether..... Aspergillus fumigatusis a major human pathogen. In contrast,Aspergillus fischeriand the recently describedAspergillus oerlinghausenensis, the two species most closely related toA. fumigatus, are not known to be pathogenic. Some of the genetic determinants of virulence (or cards of virulence) that A.fumigatuspossesses are secondary metabolites that impair the host immune system, protect from host immune cell attacks, or acquire key nutrients. To examine whether secondary metabolism-associated cards of virulence vary between these species, we conducted extensive genomic and secondary metabolite profiling analyses of multipleA. fumigatus, oneA. oerlinghausenensis, and multipleA. fischeristrains. We identified two cards of virulence (gliotoxin and fumitremorgin) shared by all three species and three cards of virulence (trypacidin, pseurotin, and fumagillin) that are variable. For example, we found that all species and strains examined biosynthesized gliotoxin, which is known to contribute to virulence, consistent with the conservation of the gliotoxin biosynthetic gene cluster (BGC) across genomes. For other secondary metabolites, such as fumitremorgin, a modulator of host biology, we found that all species produced the metabolite but that there was strain heterogeneity in its production within species. Finally, species differed in their biosynthesis of fumagillin and pseurotin, both contributors to host tissue damage during invasive aspergillosis.A. fumigatusbiosynthesized fumagillin and pseurotin, whileA. oerlinghausenensisbiosynthesized fumagillin andA. fischeribiosynthesized neither. These biochemical differences were reflected in sequence divergence of the intertwined fumagillin/pseurotin BGCs across genomes. These results delineate the similarities and differences in secondary metabolism-associated cards of virulence between a major fungal pathogen and its nonpathogenic closest relatives, shedding light onto the genetic and phenotypic changes associated with the evolution of fungal pathogenicity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据