期刊
GASTROENTEROLOGY
卷 160, 期 1, 页码 302-+出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1053/j.gastro.2020.09.040
关键词
IL-33/ST2 Axis; M2 Macrophages; SPEM/Intestinalized SPEM; Gastric Cancer
资金
- National Institutes of Health [DK056762, DK091222, DK042191, CA150964, DK071590, DK105129]
- National Research Service Award F31 Predoctoral Fellowship [DK104600]
- Institutional T32 Fellowship [DK083251]
- Cleveland Silvio O. Conte Digestive Diseases Research Core Center [DK097948]
- DeGregorio Family Foundation
- Department of Veterans Affairs [I01BX000930]
- Crohn's & Colitis Foundation [RFA326877, CDA581292, RFA410354, SRFA592800]
- American Gastroenterological Association: Eli & Edythe Broad Student Research Fellowship Award
- Italian Society of Gastroenterology
IL33 promotes the development of intestinalized spasmolytic polypeptide-expressing metaplasia (SPEM) in SAMP mice by promoting eosinophil-dependent infiltration of IL33-producing M2 macrophages, indicating that IL33 may serve as a potential therapeutic target for preneoplastic conditions of the gastrointestinal tract.
BACKGROUND & AIMS: Interleukin (IL)33/IL1F11 is an important mediator for the development of type 2 T-helper cell (Th2)-driven inflammatory disorders and has also been implicated in the pathogenesis of gastrointestinal (GI)-related cancers, including gastric carcinoma. We therefore sought to mechanistically determine IL33's potential role as a critical factor linking chronic inflammation and gastric carcinogenesis using gastritis-prone SAMP1/YitFc (SAMP) mice. METHODS: SAMP and (parental control) AKR mice were assessed for baseline gastritis and progression to metaplasia. Expression/ localization of IL33 and its receptor, ST2/IL1R4, were characterized in corpus tissues, and activation and neutralization studies were both performed targeting the IL33/ST2 axis. Dissection of immune pathways leading to metaplasia was evaluated, including eosinophil depletion studies using anti-IL5/anti-CCR3 treatment. RESULTS: Progressive gastritis and, ultimately, intestinalized spasmolytic polypeptide-expressing metaplasia (SPEM) was detected in SAMP stomachs, which was absent in AKR but could be moderately induced with exogenous, recombinant IL33. Robust peripheral (bone marrow) expansion of eosinophils and local recruitment of both eosinophils and IL33-expressing M2 macrophages into corpus tissues were evident in SAMP. Interestingly, IL33 blockade did not affect bone marrow-derived expansion and local infiltration of eosinophils, but markedly decreased M2 macrophages and SPEM features, while eosinophil depletion caused a significant reduction in both local IL33-producing M2 macrophages and SPEM in SAMP. CONCLUSIONS: IL33 promotes metaplasia and the sequelae of eosinophil-dependent downstream infiltration of IL33-producing M2 macrophages leading to intestinalized SPEM in SAMP, suggesting that IL33 represents a critical link between chronic gastritis and intestinalizing metaplasia that may serve as a potential therapeutic target for preneoplastic conditions of the GI tract.
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