期刊
FOOD CHEMISTRY
卷 324, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126832
关键词
Aflatoxin B1 (AFB1); Peanut oil; Surface-enhanced Raman spectroscopy (SERS); Aptamer; Capture probes; Reporter probes; 4-mercaptobenzoic acid (4-MBA)
资金
- National Key RAMP
- D Program of China [2018YFC1603400]
- Contemporary International Collaborative Research Centre of Guangdong Province on Food Innovative Processing and Intelligent Control [2019A050519001]
- Common Technical Innovation Team of Guangdong Province on Preservation and Logistics of Agricultural Products [2019KJ145, 2019KJ101]
- Innovation Centre of Guangdong Province for Modern Agricultural Science and Technology on Intelligent Sensing and Precision Control of Agricultural Product Qualities
- Key Research and Development Program of Guangdong Province [2019B020212001]
Aflatoxin B1 (AFB1) as the most toxic mycotoxin in contaminated food can greatly threaten human health, and sensitive and selective detection of AFB1 is thus highly desired. An ultrasensitive surface-enhanced Raman spectroscopy (SERS) aptasensor was developed for AFB1 detection in peanut oil samples. SH-cDNA modified Fe3O4@Au nanoflowers acted as capture probes, SH-Apt modified Au@Ag nanospheres and commercial Cy3-Apt were used as reporter probes. Strong SERS signals of reporter probes were produced due to the recognition of AFB1 aptamer and its complementary strand (SH-cDNA). With the preferred binding of AFB1 aptamer to AFB1, reporter probes were released from capture probes, causing a linear decrease in SERS intensity. Therefore an ultralow detection limit of 0.40 pg.mL(-1) in a wide linear range of 0.0001-100 ng.mL(-1) was obtained and the sensibility of this SERS aptasensor was higher than that of the Cy3-Apt based SERS aptasensor. In addition, an excellent selectivity in interfering toxins and satisfactory recoveries of 96.6-115% in peanut oil samples were obtained, proving this aptasensor is a promising analytical tool in AFB1 detection.
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