期刊
FISH & SHELLFISH IMMUNOLOGY
卷 112, 期 -, 页码 168-178出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.08.050
关键词
Ctenopharyngodon idellus; Heme oxygenase-1; Anti-inflammatory; Immune response
资金
- National Natural Science Foundation of China [31902409, 31872606, 31572657, U1701233]
- Foundation of Guangdong Provincial Marine and Fisheries Bureau [GDME2018C006, D21822202]
- Foundation of China-ASEAN Maritime Cooperation [CAMC2018F]
- Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams [2019KJ141]
- Pearl River Scholarship from Guangdong Province
This study cloned and characterized the HO-1 gene of grass carp, showing that GcHO-1 is widely expressed in healthy grass carp and significantly up-regulated in the liver and head-kidney tissues after infection of Staphylococcus aureus and Aeromonas hydrophila. Overexpression of GcHO-1 in kidney cell line cells could reduce the expression of inflammatory cytokines, indicating an anti-inflammatory role in innate immunity.
Heme oxygenase (HO)-1, a rate-limiting enzyme in heme catabolism, results in the formation of equivalent amounts of biliverdin (BV), carbon monoxide (CO) and ferrous iron (Fe2+). Previous studies have revealed that HO-1 plays an important role in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost HO-1 remains enigmatic. To decipher the mechanisms, we have cloned and characterized the HO-1 gene of grass carp (designated as GcHO-1) in this research. The results showed that the open reading frame (ORF) of GcHO-1 was 819 bp, which encoded a putative protein of 272 amino acids. The deduced amino acid sequence phylogenetically shared the highest identity with other teleosts, and contained two domains of heme-oxygenase and a single-pass transmembrane domain. The mRNA expressions of GcHO-1 in healthy grass carp have widely existed in examined tissues in the following order of spleen > head-kidney > middle head-kidney > intestines > liver > gills > heart > muscle > brain. Besides, the mRNA and protein transcription of GcHO-1 were both significantly up-regulated in the liver and head-kidney tissues after Staphylococcus aureus and Aeromonas hydrophila infection. In addition, overexpression of GcHO-1 in kidney cell line (CIK) cells of grass carp could reduce the expression of inflammatory cytokines (IL-1?, IL-8, TNF?, CCL1 and IL6). Herein, we demonstrate that GcHO-1 plays an anti-inflammatory role in innate immunity. Our results shed new light on the mechanisms underlying the antibacterial immunity of teleost.
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