期刊
FEBS JOURNAL
卷 288, 期 14, 页码 4230-4241出版社
WILEY
DOI: 10.1111/febs.15606
关键词
extranasal expression; NGS; odorant receptors; olfaction; single cells
资金
- Ramalingaswami Re-entry Fellowship, a re-entry scheme of the Department of Biotechnology (DBT), Ministry of Science & Technology, Govt. of India
- Indraprastha Institute of Information Technology-Delhi (IIIT-Delhi)
- INSPIRE faculty grant from the Department of Science & Technology (DST), India
High-throughput omics technologies have revealed the expression of olfactory receptors in nonolfactory tissues, but lack information about their cell type of origin. Single-cell RNA-sequencing technology addresses this issue and provides a new means for cell type-specific analysis, while large-scale single-cell expression atlases offer resources for systematic reannotation of extranasal olfactory receptors.
Olfactory receptors are primarily known to be expressed in the olfactory epithelium of the nasal cavity and therefore assist in odor perception. With the advent of high-throughput omics technologies such as tissue microarray or RNA sequencing, a large number of olfactory receptors have been reported to be expressed in the nonolfactory tissues. Although these technologies uncovered the expression of these olfactory receptors in the nonchemosensory tissues, unfortunately, they failed to reveal the information about their cell type of origin. Accurate characterization of the cell types should be the first step towards devising cell type-specific assays for their functional evaluation. Single-cell RNA-sequencing technology resolved some of these apparent limitations and opened new means to interrogate the expression of these extranasal olfactory receptors at the single-cell resolution. Moreover, the availability of large-scale, multi-organ/species single-cell expression atlases offer ample resources for the systematic reannotation of these receptors in a cell type-specific manner. In this Viewpoint article, we discuss some of the technical limitations that impede the in-depth understanding of these extranasal olfactory receptors, with a special focus on odorant receptors. Moreover, we also propose a list of single cell-based omics technologies that could further promulgate the opportunity to decipher the regulatory network that drives the odorant receptors expression at atypical locations.
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