期刊
EXPERIMENTAL DERMATOLOGY
卷 30, 期 1, 页码 62-67出版社
WILEY
DOI: 10.1111/exd.14196
关键词
atypical PKC; cell polarity; COL17; epidermal stem cell; PAR3
类别
资金
- Japan Agency for Medical Research and Development [18059057]
- Uehara Memorial Foundation
COL17 is crucial for maintaining epidermal cell polarity, as its deficiency can lead to asymmetric cell division and affect the health of epidermal cells.
Type XVII collagen (COL17) is a transmembrane protein expressed in the basal epidermis. COL17 serves as a niche for epidermal stem cells, and although its reduction has been implicated in altering cell polarity and ageing of the epidermis, it is unknown how COL17 affects epidermal cell polarity. Here, we uncovered COL17 as a binding partner of the aPKC-PAR complex, which is a key regulating factor of cell polarity. Immunoprecipitation-immunoblot assay and protein-protein binding assay revealed that COL17 interacts with aPKC and PAR3. COL17 deficiency or epidermis-specific aPKC lambda deletion destabilized PAR3 distribution in the epidermis, while aPKC zeta knockout did not. Asymmetrical cell division was pronounced in COL17-null neonatal paw epidermis. These results show that COL17 is pivotal for maintaining epidermal cell polarity. Our study highlights the previously unrecognized role of COL17 in the basal keratinocytes.
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