期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 890, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ejphar.2020.173649
关键词
Gekko japonicus; Cathelicidin; Truncated peptide; Antioxidant; Wound healing
资金
- National Natural Science Foundation of China [31900328]
- National Natural Science Foundation Cultivation Project of Jining Medical University [JYP2018KJ01]
- Doctoral Scientific Research Foundation of Jining Medical University
A novel Cathelicidin, Gj-CATH3, was identified from Gekko japonicas, showing potent antioxidant activity. Through designing analogues, two truncated peptides demonstrated strong antioxidant activity and significant wound healing properties, with decreased cytotoxicity and hemolytic activities compared to Gj-CATH3.
Cathelicidins are a class of gene-encoded multifunctional factors in host defence systems. They have recently attracted a great deal of attention as promising drug candidates. Cathelicidins are well studied in vertebrates, yet no studies have been reported concerning gecko cathelicidin. Recently, we identified a novel cathelicidin from Gekko japonicas, Gj-CATH3. Unlike most cathelicidins, Gj-CATH3 exhibits potent antioxidant activity in vitro. Unfortunately, slight toxicity and high synthesis cost restrict its application. Thus, we designed a series of GjCATH3 analogues for development of short peptides with improved cell selectivity. Functional analysis showed that two truncated peptides, Gj-CATH3-(38-42)-peptide and Gj-CATH3-(33-42)-peptide, exhibited excellent antioxidant activity against ABTS and DPPH free radicals. Further, cytotoxicity and hemolytic activities were observably lower compared to Gj-CATH3. Interestingly, both peptides also demonstrate significant wound healing properties in a mouse model with full-thickness skin wounds. The peptides induce HaCaT cell proliferation and prevent decreases in SOD activity and increases of MDA concentration in injured-skin tissue. This report is the first to address cathelicidin from reptilia that exhibit potent wound healing activity. Our research will enrich understanding of cathelicidin biological functions, and provide a theoretical basis for its clinical application.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据