期刊
EQUINE VETERINARY JOURNAL
卷 53, 期 4, 页码 834-844出版社
WILEY
DOI: 10.1111/evj.13356
关键词
horse; supporting limb; laminitis; ischaemia; microdialysis; bioenergetics; perfusion
资金
- Grayson Jockey Club Research Foundation
The study demonstrated that lamellar ischemia, characterized by increased L:P ratio and decreased urea clearance, was detected exclusively in the lamellar dermis of feet subjected to prolonged increased load, indicating a potential association between lamellar ischemia and the development of supporting limb laminitis (SLL).
Background Supporting limb laminitis (SLL) is suspected to be caused by lamellar ischaemia as a consequence of increased mechanical load. Objectives Examine the effects of prolonged preferential weight bearing (PWB) on lamellar perfusion and metabolism. Study design In vivo experiment. Methods Microdialysis probes were inserted in the lamellar and sublamellar dermis of one forelimb in 13 Standardbred horses. In six horses, a platform shoe (contralateral forelimb) was used to induce increased load on the microdialysis-instrumented forelimb (PWB). The remaining seven horses were controls (CON). All horses were housed in stocks with limb weight distribution logged continuously for 92 hours. Microdialysate was collected and analysed every 4 hours for glucose, lactate, pyruvate, and lactate to pyruvate ratio (L:P). Microdialysis urea clearance was used to estimate lamellar perfusion. Data were analysed using a mixed-effects linear regression model. Results Median [IQR] load on the microdialysis-instrumented limb was equivalent to 38.7% bwt. [37.3-40.3] in PWB and 27.3% bwt. [26.6-28] in CON. Limb offloading frequency increased in CON (P < .001) but not PWB (P = .2). Lamellar microdialysate glucose decreased in PWB (P < .001) and CON (P = .004), however, the rate of decrease was higher in PWB (P = .007). Lamellar L:P increased in PWB (P < .001) and peaked at 196 [79-656], whereas L:P did not change over time in CON (P = .6) and peaked at 42 [41-49]. Lamellar urea clearance decreased in PWB (P < .001) but not in CON (P = .3). Sublamellar L:P and urea clearance did not change over time in either group (P > .05). Main limitations The PWB model may not be representative of naturally occurring SLL. Conclusions Evidence of lamellar ischaemia (increased L:P and decreased urea clearance) was detected exclusively in the lamellar dermis of PWB feet subjected to persistently increased load. Lamellar ischaemia is a consequence of increased mechanical load and likely contributes to the development of SLL.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据