Abnormal steroidogenesis, oxidative stress, and reprotoxicity following prepubertal exposure to butylparaben in mice and protective effect ofCurcuma longa

Mammalian reproduction is a highly regulated process that can be distorted following exposure to synthetic antimicrobial preservatives like butylparaben (BP). Besides, studies have not investigated the potential antioxidant effects of turmeric on BP-provoked reprotoxicity. The present research was planned on prepubertal mice, orally treated with BP (150 mu g/g body weight/day) with and withoutCurcuma longa(turmeric) (400 mu g/mice/day) from postnatal day 35 to 65 routinely. Results showed an insignificant reduction in body weight of both sexes but contrary to these, gonadal weight increased significantly in PB-exposed mice. Additionally, elevated levels of follicle-stimulating hormone and luteinizing hormone while decreased estrogen levels were observed in BP-treated females against control. Sperm count and motility were disturbed, coupled with abnormal sperm morphology in BP-intoxicated group. These findings were synchronized with a decreased testosterone levels in the same group as compared with control. The follicular count revealed reduction in the number of antral follicles while an increase in empty follicles. The BP also significantly increased lipid peroxidation and decreased glutathione content, superoxide dismutase, and catalase activities, while the morphometric, biochemical, and histological deviations were less pronounced in the group, which was co-administered with BP and turmeric. Results indicated that turmeric has antioxidant potential to protect BP-induced oxidative stress and reprotoxicity in mice.

Automated summary

The study found that exposure to BP led to stable body weight in mice, but a significant increase in gonadal weight, elevated levels of follicle-stimulating hormone and luteinizing hormone, decreased estrogen levels in treated females, disrupted sperm count and motility, abnormal sperm morphology, decreased testosterone levels, reduced number of antral follicles and increased lipid peroxidation. Co-administration of BP and turmeric resulted in fewer biochemical and histological deviations, indicating turmeric's potential antioxidant effects against BP-induced oxidative stress and reprotoxicity in mice.