Biogenic fenton-like reaction involvement in aerobic degradation of C60 by Labrys sp. WJW

Buckminster fullerene (C-60), the most representative type among fullerenes, has attracted widely attentions because of its many potential applications. The increasing application of fullerene and limited knowledge of its environmental fate are required concerns. Herein, the biotransformation of C-60 by Labrys sp. WJW was investigated. Cell numbers reached 25.76 +/- 1.85 folds within 8 days using 100 mg/L C-60 as sole carbon source. The biotransformation of C-60 by Labrys sp. WJW was analyzed by various characterization methods. Raman spectra indicated that strain WJW broke the soccer ball like structure of C-60. After 12 days, over 60% of C-60 was degraded evidenced by UV-vis spectrophotometry and liquid chromatography-mass spectrometry. The underlying biotransformation mechanism of C-60 through an extracellular Fenton-like reaction was illustrated. In this reaction, the (OH)-O-center dot production was mediated by reduction of H2O2 involving a continuous cycle of Fe(II)/Fe(III). Bacterial transformation of C-60 will provide new insights into the understanding of C-60 bioremediation process. (C) 2020 Published by Elsevier Ltd.

Automated summary

This study investigated the biotransformation of C-60 by Labrys sp. WJW, showing that the strain could effectively degrade C-60 and uncovering the biotransformation mechanism, providing new insights into the bioremediation of C-60.