期刊
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
卷 169, 期 2, 页码 156-171出版社
WILEY
DOI: 10.1111/eea.12982
关键词
Heliothis virescens; Helicoverpa armigera; sex pheromone; communication; odorant receptor; heterologous expression system; ligand selectivity; Noctuidae; Lepidoptera; Heliothinae; devastating pest species; olfactory receptor neuron; CRISPR/Cas9
类别
资金
- National Natural Science Foundation of China [31772528, 31830088]
- China Postdoctoral Science Foundation [2019M660792]
This article focuses on functional characterization of pheromone receptors (PRs) in heliothine species, summarizing recent progress in identification of receptors tuned to principal sex pheromone components. The efficiency and comparison of three frequently used heterologous expression systems are discussed, along with research strategies and future research topics on PRs in heliothine species.
Heliothine species (Lepidoptera: Noctuidae, Heliothinae) include some of the world's most devastating pest species, such as Heliothis virescens (Fabricius) in the Americas and Helicoverpa armigera (Hubner) in the Old World. Sex pheromone communication of these agricultural pest species has been investigated from pheromone receptors (PRs) to brains and behavior in detail. In recent years, great progress has been made in the mechanistic dissection of pheromone reception, demonstrating that PRs play a key role in determining the response characteristics of pheromone-sensitive olfactory receptor neurons. In this review, we focus on the functional characterization of PRs in heliothine species, and summarize recent progress in the identification of receptors tuned to principal sex pheromone components including Z11-16:Ald, Z9-16:Ald, Z9-14:Ald, and other related alcohols and acetates. Evolution of PRs in ligand selectivity is also discussed. The efficiency and faithfulness of three frequently used heterologous expression systems - Xenopus laevis Daudin oocytes, modified HEK 293 cells, and Drosophila 'empty neuron' mutants - are compared, and the research strategies, skills, and perspectives in the studies are envisioned. Finally, we propose future research topics on PRs in heliothine species.
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