期刊
BMC GENOMICS
卷 16, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s12864-015-1505-5
关键词
Trypanosoma brucei; RNA interference; Cell cycle; Mitochondrion; Kinetoplast
资金
- MIE (Emerging Infectious Diseases) program of CNRS
- University Montpellier 1 (Bonus Qualite Recherche)
- Agence Nationale de la Recherche within Investissements d'avenir program (ParaFrap) [ANR-11-LABX-0024-01]
- French Ministry of Research
- Wellcome Trust
- Fondation Infectiopole Sud
- Centre Hospitalier Universitaire of Montpellier
Background: Trypanosomatid parasites possess a single mitochondrion which is classically involved in the energetic metabolism of the cell, but also, in a much more original way, through its single and complex DNA (termed kinetoplast), in the correct progress of cell division. In order to identify proteins potentially involved in the cell cycle, we performed RNAi knockdowns of 101 genes encoding mitochondrial proteins using procyclic cells of Trypanosoma brucei. Results: A major cell growth reduction was observed in 10 cases and a moderate reduction in 29 other cases. These data are overall in agreement with those previously obtained by a case-by-case approach performed on chromosome 1 genes, and quantitatively with those obtained by high-throughput phenotyping using parallel sequencing of RNA interference targets (RIT-seq). Nevertheless, a detailed analysis revealed many qualitative discrepancies with the RIT-seq-based approach. Moreover, for 37 out of 39 mutants for which a moderate or severe growth defect was observed here, we noted abnormalities in the cell cycle progress, leading to increased proportions of abnormal cell cycle stages, such as cells containing more than 2 kinetoplasts (K) and/or more than 2 nuclei (N), and modified proportions of the normal phenotypes (1N1K, 1N2K and 2N2K). Conclusions: These data, together with the observation of other abnormal phenotypes, show that all the corresponding mitochondrial proteins are involved, directly or indirectly, in the correct progress or, less likely, in the regulation, of the cell cycle in Trypanosoma brucei. They also show how post-genomics analyses performed on a case-by-case basis may yield discrepancies with global approaches.
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