期刊
EMBO JOURNAL
卷 39, 期 21, 页码 -出版社
WILEY
DOI: 10.15252/embj.2020106003
关键词
angiogenesis; endothelial cell; filopodia; mRNA targeting; zebrafish
资金
- Wellcome Trust [095718/Z/11/Z, 219500/Z/19/Z]
- Wellcome Institutional Strategic Support Fund [204796/Z/16/Z]
- British Heart Foundation [PG/16/2/31863]
- Wellcome Trust [219500/Z/19/Z, 095718/Z/11/Z] Funding Source: Wellcome Trust
Polarised targeting of diverse mRNAs to cellular protrusions is a hallmark of cell migration. Although a widespread phenomenon, definitive functions for endogenous targeted mRNAs and their relevance to modulation ofin vivotissue dynamics remain elusive. Here, using single-molecule analysis, gene editing and zebrafish live-cell imaging, we report that mRNA polarisation acts as a molecular compass that orients motile cell polarity and spatially directs tissue movement. Clustering of protrusion-derived RNAseq datasets defined a core 192-nt localisation element underpinning precise mRNA targeting to sites of filopodia formation. Such targeting of the small GTPase RAB13 generated tight spatial coupling of mRNA localisation, translation and protein activity, achieving precise subcellular compartmentalisation of RAB13 protein function to create a polarised domain of filopodia extension. Consequently, genomic excision of this localisation element and perturbation ofRAB13mRNA targeting-but not translation-depolarised filopodia dynamics in motile endothelial cells and induced mispatterning of blood vessels in zebrafish. Hence, mRNA polarisation, not expression, is the primary determinant of the site of RAB13 action, preventing ectopic functionality at inappropriate subcellular loci and orienting tissue morphogenesis.
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