期刊
ELECTROPHORESIS
卷 41, 期 20, 页码 1804-1811出版社
WILEY
DOI: 10.1002/elps.202000201
关键词
Gas chromatography-mass spectrometry (GC-MS); Inositols; Lettuce (Latuca sativa); Microwave-assisted extraction; Valorization
资金
- Ministerio de Economia, Industria y Competitividad (AEI/FEDER, UE) [AGL2016-80475-R]
- CSIC [202080E216]
- Comunidad of Madrid
- FSE program (AVANSECAL-II-CM) [S2018/BAA-4393]
- FEDER program (AVANSECAL-II-CM) [S2018/BAA-4393]
- Universidad del Cauca of Colombia [501100005682, 4486]
A microwave-assisted extraction (MAE) method was developed for the extraction of bioactive inositols (D-chiro- andmyo-inositols) from lettuce (Lactuca sativa) leaves as a strategy for the revalorization of these agrofood residues. Gas chromatography-mass spectrometry was selected for the simultaneous determination of inositols and sugars (glucose, fructose, and sucrose) in these samples. A Box-Behnken experimental design was used to maximize the extraction of inositols based on the results of single factor tests. Optimal conditions of the extraction process were as follows: liquid-to-solid ratio of 100:1 v/w, 40 degrees C, 30 min extraction time, 20:80 ethanol:water (v/v), and one extraction cycle. When compared with conventional solid-liquid extraction (SLE), MAE was found to be more effective for the extraction of target bioactive carbohydrates (MAE 5.42 mg/g dry sample versus SLE 4.01 mg/g dry sample). Then, MAE methodology was applied to the extraction of inositols fromL. sativaleaves of different varieties (var.longifolia, var.capitataand var.crispa). D-chiro- andmyo-inositol contents varied between 0.57-7.15 and 0.83-3.48 mg/g dry sample, respectively. Interfering sugars were removed from the extracts using a biotechnological procedure based on the use ofSaccharomyces cerevisiaefor 24 h. The developed methodology was a good alternative to classical procedures to obtain extracts enriched in inositols from lettuce residues, which could be of interest for the agrofood industry.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据