期刊
ELECTROANALYSIS
卷 33, 期 1, 页码 216-225出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elan.202060256
关键词
3-Nitro-tyrosine; Oxidation; 3-Nitro-tyrosine-DNA interaction; Voltammetry; Agarose gel electrophoresis
资金
- Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE) [PPP/FACEPE/CNPq/APQ-0535-1.06/14]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [MCTI/CNPQ/Universal/APQ-422757/2018-7]
- FACEPE
- CAPES
The electrochemical oxidation of 3-nitro-tyrosine at metallic electrodes was studied, revealing a complex process with strong interaction of 3-NO2-Tyr with dsDNA.
The electrochemical oxidation of 3-nitro-tyrosine (3-NO2-Tyr) was studied in aqueous media at metallic electrodes (platinum and gold), using voltammetric techniques. The interaction between 3-NO2-Tyr and double helix DNA (dsDNA) in a physiological medium was also investigated. Electro-oxidation of 3-NO2-Tyr occurs in one single irreversible pH-dependent step with the transfer of one electron and one proton from the phenolic group to the formation of radicals, which preferably dimerize, fouling the electrode surfaces. The differential pulse voltammetry and gel electrophoresis results clearly demonstrated a strong interaction of 3-NO2-Tyr with the dsDNA for the formation of a stable 3-NO2-Tyr-dsDNA complex.
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